Simultaneous quantification of three alkylatedpurine adducts in human urine using sulfonic acid poly(glycidyl methacrylatedivinylbenzene)-based microspheres as sorbent combined with LC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1081-1082: 15-24, 2018 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-29499464
ABSTRACT
Three alkylated DNA adducts, N3methyladenine, N3ethyladenine and N7ethylguanine, have been proved to be potential biomarkers for DNA injury caused by exposure to cigarette smoke. In this study, a highly specific and sensitive method using a new mixed-mode sulfonate-functionalized poly(glycidyl methacrylate-divinylbenzene) as a solid-phase extraction sorbent was developed for the analysis of these three alkylated-purine adducts in human urine. Under optimized conditions, the prepared sorbent interacts strongly with these urinary adducts, demonstrating high clean-up efficiency and extraction recovery. The method detection limits (S/Nâ¯≥â¯3) of N3-MeA, N3-EtA and N7-EtG were 1.75, 0.20, and 0.15â¯pgâ¯mL-1, respectively, while the method quantitation limits were found to be 5.78, 0.66, and 0.49â¯pgâ¯mL-1 for N3-MeA, N3-EtA and N7-EtG, respectively. The intra-day and inter-day precisions were investigated, of which were in the range of 1.6-3.8% and 3.2-5.6%, respectively. The recovery values of the alkylated DNA adducts in spiked urine sample were ranged 89.7-104.5%. Their concentrations were statistically significantly higher in smokers than in nonsmokers. These results show that the proposed method is suitable for the analysis of alkylated DNA adducts.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Purines
/
Chromatography, Liquid
/
DNA Adducts
/
Tandem Mass Spectrometry
/
Microspheres
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2018
Document type:
Article
Affiliation country:
China