Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification.
Curr Protoc Microbiol
; 48: 15M.1.1-15M.1.14, 2018 02 22.
Article
in En
| MEDLINE
| ID: mdl-29512113
ABSTRACT
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirale. PRRSV is an enveloped, single-stranded, positive-sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC-145 cell line is the most often used in a laboratory setting. Infectious cDNA clones of many PRRSV strains have been established, from which these viruses can be recovered. PRRSV titration is generally done in MARC-145 cells. PRRSV RNA copy numbers can be assessed by reverse transcription and real-time PCR. Here, protocols for PRRSV propagation, virus recovery from infectious cDNA clones, and quantification are presented. © 2018 by John Wiley & Sons, Inc.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Cultivation
/
Polymerase Chain Reaction
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Cell Culture Techniques
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Porcine respiratory and reproductive syndrome virus
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Porcine Reproductive and Respiratory Syndrome
Limits:
Animals
Language:
En
Journal:
Curr Protoc Microbiol
Year:
2018
Document type:
Article