Your browser doesn't support javascript.
loading
Isolation of human mesenchymal stem cells from amnion, chorion, placental decidua and umbilical cord: comparison of four enzymatic protocols.
Araújo, A B; Furlan, J M; Salton, G D; Schmalfuss, T; Röhsig, L M; Silla, L M R; Passos, E P; Paz, A H.
Affiliation
  • Araújo AB; Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil. anelise_araujo@yahoo.com.br.
  • Furlan JM; Federal University of Rio Grande do Sul, Porto Alegre, Brazil. anelise_araujo@yahoo.com.br.
  • Salton GD; Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil. anelise_araujo@yahoo.com.br.
  • Schmalfuss T; Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
  • Röhsig LM; Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
  • Silla LMR; Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
  • Passos EP; Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
  • Paz AH; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, 90035-903, Porto Alegre, Rio Grande do Sul, Brazil.
Biotechnol Lett ; 40(6): 989-998, 2018 Jun.
Article in En | MEDLINE | ID: mdl-29619744
OBJECTIVE: To compare four enzymatic protocols for mesenchymal stem cells (MSCs) isolation from amniotic (A-MSC) and chorionic (C-MSC) membranes, umbilical cord (UC-MSC) and placental decidua (D-MSC) in order to define a robust, practical and low-cost protocol for each tissue. RESULTS: A-MSCs and UC-MSCs could be isolated from all samples using trypsin/collagenase-based protocols; C-MSCs could be isolated from all samples with collagenase- and trypsin/collagenase-based protocols; D-MSCs were isolated from all samples exclusively with a collagenase-based protocol. CONCLUSIONS: The trypsin-only protocol was least efficient; the collagenase-only protocol was best for C-MSCs and D-MSCs; the combination of trypsin and collagenase was best for UC-MSCs and none of tested protocols was adequate for A-MSCs isolation.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Umbilical Cord / Cell Separation / Extraembryonic Membranes / Mesenchymal Stem Cells Type of study: Guideline Limits: Female / Humans / Pregnancy Language: En Journal: Biotechnol Lett Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Netherlands
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Umbilical Cord / Cell Separation / Extraembryonic Membranes / Mesenchymal Stem Cells Type of study: Guideline Limits: Female / Humans / Pregnancy Language: En Journal: Biotechnol Lett Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Netherlands