Induced Pluripotent Stem Cells Promote Retinal Ganglion Cell Survival After Transplant.
Invest Ophthalmol Vis Sci
; 59(3): 1571-1576, 2018 03 01.
Article
in En
| MEDLINE
| ID: mdl-29625481
ABSTRACT
Purpose:
The purpose of this study was to characterize whether induced pluripotent stem cells (iPSCs) affect survival of grafted retinal ganglion cells (RGCs) after transplantation.Methods:
For in vitro studies, human iPSCs were either directly cocultured with mouse RGCs or plated in hanging inserts in RGC cultures for 1 week. For ex vivo studies, RGCs and iPSCs were seeded onto the inner surface of an adult rat retina explant and cultured for 1 week. For in vivo studies, RGCs and iPSCs were intravitreally coinjected into an adult rat eye 1 week before examining retinas by explant and immunostaining.Results:
A dose-dependent increase in RGC survival was observed in RGC-iPSC direct cocultures, and RGC-iPSC indirect cocultures showed a similar RGC protective effect, but to a lesser extent than in direct coculture. Enhanced RGC survival was also identified in RGC-iPSC cotransplantations to adult retinas ex vivo and in vivo. In addition, RGCs with iPSC cotransplantation extended significantly longer neurites than RGC-only transplants.Conclusions:
Human iPSCs promote transplanted RGC survival and neurite extension. This effect may be mediated at least partially through secretion of diffusible neuroprotective factors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retina
/
Retinal Ganglion Cells
/
Induced Pluripotent Stem Cells
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Invest Ophthalmol Vis Sci
Year:
2018
Document type:
Article