A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii.

Goda, Shuichiro; Koga, Tomoyuki; Yamashita, Kenichiro; Kuriura, Ryo; Ueda, Toshifumi

Goda, Shuichiro; Koga, Tomoyuki; Yamashita, Kenichiro; Kuriura, Ryo; Ueda, Toshifumi.

Affiliation

Goda S; a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.
Koga T; a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.
Yamashita K; a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.
Kuriura R; a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.
Ueda T; a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.

Biosci Biotechnol Biochem ; 82(8): 1327-1334, 2018 Aug.

Article in En | MEDLINE | ID: mdl-29629656

Subject(s)

Acetylglucosamine/metabolism; Archaeal Proteins/metabolism; Pyrococcus horikoshii/metabolism; Amino Acid Sequence; Archaeal Proteins/chemistry; Archaeal Proteins/genetics; Archaeal Proteins/isolation & purification; Calorimetry/methods; Chromatography, Affinity/methods; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Genes, Archaeal; Green Fluorescent Proteins/metabolism; Hot Temperature; Micrococcus/metabolism; Protein Binding; Protein Conformation; Protein Denaturation; Protein Stability; Scattering, Small Angle; X-Ray Diffraction

Key words

Archaea; GlcNAc: N-acetylglucosamine; ITC: isothermal titration calorimetry; Pyrococcus horikoshii; S-layer protein: surface-layer protein; TBS: Tris-buffered saline; carbohydrate binding; surface-layer protein

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acetylglucosamine / Archaeal Proteins / Pyrococcus horikoshii Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2018 Document type: Article Affiliation country: Japan Country of publication: United kingdom

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