Two-photon oxygen nanosensors based on a conjugated fluorescent polymer doped with platinum porphyrins.
Methods Appl Fluoresc
; 6(3): 035008, 2018 Apr 27.
Article
in En
| MEDLINE
| ID: mdl-29651992
ABSTRACT
Ratiometric fluorescent nanoparticles (NPs) under two-photon excitation are successfully developed for sensing dissolved oxygen. The NPs comprise the oxygen probe Pt(II)-porphyrins (PtTFPP) and fluorescent organic semiconducting polymer (PFO). PFO polymer acts as both a two-photon antenna and a reference dye, while PtTFPP absorbs the photonic energy transferred by the PFO under two-photon excitation at 740 nm to sense oxygen. The red fluorescence of PtTFPP is sensitive to oxygen with a quenching response of 88% from nitrogen saturation to oxygen saturation, and PFO gives oxygen-insensitive referenced blue fluorescence. The fluorescence quenching of the NPs against oxygen at two-photon excitation follows a linear Stern-Volmer behavior. The nanosensors exhibit low cytotoxic effects as well as effortless cellular uptake. When incorporated into cells, the ratio of the signals increases up to about 500% from oxygen-saturated to oxygen-free environment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Platinum
/
Polymers
/
Porphyrins
/
Nanoparticles
Limits:
Humans
Language:
En
Journal:
Methods Appl Fluoresc
Year:
2018
Document type:
Article