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Pharmacokinetics and pharmacodynamic effects in koi carp (Cyprinus carpio) following immersion in propofol.
Oda, Ayako; Messenger, Kristen M; Carbajal, Liliana; Posner, Lysa P; Gardner, Brett R; Hammer, Scott H; Cerreta, Anthony J; Lewbart, Gregory A; Bailey, Kate M.
Affiliation
  • Oda A; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. Electronic address: aoda@ncsu.edu.
  • Messenger KM; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
  • Carbajal L; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
  • Posner LP; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
  • Gardner BR; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, College of Veterinary Medicine, Raleigh, NC, USA.
  • Hammer SH; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
  • Cerreta AJ; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
  • Lewbart GA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, College of Veterinary Medicine, Raleigh, NC, USA.
  • Bailey KM; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Vet Anaesth Analg ; 45(4): 529-538, 2018 Jul.
Article in En | MEDLINE | ID: mdl-29705687
OBJECTIVE: To test the hypothesis that plasma propofol concentration (PPC) is associated with anesthetic effect in koi carp administered propofol by immersion. STUDY DESIGN: Prospective study. ANIMALS: Twenty mature koi carp (mean ± standard deviation, 409.4 ± 83.7 g). METHODS: Fish were immersed in propofol (5 mg L-1). Physiological variables and induction and recovery times were recorded. In phase I, blood was sampled for PPC immediately following induction and at recovery. In phase II, following induction, fish were maintained with propofol (4 mg L-1) via a recirculating system for 20 minutes. Following established induction, blood was sampled at 1, 10 and 20 minutes. In phase III (n = 19), fish were anesthetized as in phase II with blood sampled nine times in a sparse sampling strategy. Simultaneously, a pharmacodynamics rubric was used to evaluate anesthetic depth. PPC was determined using high performance liquid chromatography with fluorescence detection. Following evaluation of normality, data were analyzed using paired t test or Spearman correlation test (significance was set at p < 0.05). RESULTS: In phase I, mean PPCs at induction (20.12 µg mL-1) and recovery (11.62 µg mL-1) were different (p < 0.001). In phase II, only mean PPCs at induction (17.92 µg mL-1) and 10 minutes (21.50 µg mL-1) were different (p = 0.013). In phase III, a correlation between PPCs and the pharmacodynamic rubric scores was found (p < 0.001, r = -0.93). There was no correlation between PPCs and recovery time (p = 0.057, r = 0.433). A two-compartment open model was chosen for the pharmacokinetic model. Absorption rate constant, elimination rate constant and intercompartmental rate constant were 0.48, 0.006 and 0.02 minute-1, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Measurable PPCs were achieved in koi carp anesthetized with propofol by immersion. Anesthetic depth of fish was negatively correlated with PPCs, but recovery time was not.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carps / Propofol / Hypnotics and Sedatives Type of study: Observational_studies / Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Vet Anaesth Analg Journal subject: ANESTESIOLOGIA / MEDICINA VETERINARIA Year: 2018 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carps / Propofol / Hypnotics and Sedatives Type of study: Observational_studies / Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Vet Anaesth Analg Journal subject: ANESTESIOLOGIA / MEDICINA VETERINARIA Year: 2018 Document type: Article Country of publication: United States