Responses of rainbow trout intestinal epithelial cells to different kinds of nutritional deprivation.
Fish Physiol Biochem
; 44(4): 1197-1214, 2018 Aug.
Article
in En
| MEDLINE
| ID: mdl-29754319
ABSTRACT
In order to develop an in vitro system to study the cell biology of starvation in the fish intestine, rainbow trout intestinal epithelial cells were subjected to three kinds of nutrient deprivation and evaluated for 7 days. The RTgutGC cell line was grown into monolayers in Leibovitz's basal medium supplemented with fetal bovine serum (L15/FBS) and then subjected to deprivation of serum (L15); of serum, amino acids, and vitamin (L15/ex); and of all nutrients (L15/salts). After 7 days of nutrient deprivation, the cells remained attached to the plastic surface as monolayers but changes were seen in shape, with the cells becoming more polygonal, actin and α-tubulin cytoskeleton organization, and in tight junction protein-1 (ZO-1) localization. Two barrier functions, transepithelial electrical resistance (TEER) and Lucifer Yellow (LY) retention, were impaired by nutrient deprivation. In L15/FBS, cells rapidly healed a gap or wound in the monolayer. In L15 and L15/ex, some cells moved into the gap, but after 7 days, the wound remained unhealed, whereas in L15/salts, cells did not even migrate into the gap. Upon nutrient replenishment (L15/FBS) after 7 days in L15, L15/ex, or L15/salts, cells proliferated again and healed a wound. After 7 days of nutrient deprivation, monolayers were successfully passaged with trypsin and cells in L15/FBS grew to again form monolayers. Therefore, rainbow trout intestinal epithelial cells survived starvation, but barrier and wound healing functions were impaired.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oncorhynchus mykiss
/
Malnutrition
/
Epithelial Cells
/
Fish Diseases
/
Intestinal Mucosa
Aspects:
Determinantes_sociais_saude
Limits:
Animals
Language:
En
Journal:
Fish Physiol Biochem
Year:
2018
Document type:
Article
Affiliation country:
Canada