T cells and ILC2s are major effector cells in influenza-induced exacerbation of allergic airway inflammation in mice.
Eur J Immunol
; 49(1): 144-156, 2019 01.
Article
in En
| MEDLINE
| ID: mdl-29762870
ABSTRACT
Influenza virus infection is an important cause of severe asthma exacerbations, but it remains unclear how a Th1-mediated antiviral response triggers a prototypical Th2 disease. We investigated CD4+ T cells and group 2 innate lymphoid cells (ILC2s) in influenza virus-infected mice. We found that ILC2s accumulated in the lung rapidly after influenza virus infection, but the induction of IL-5 and IL-13 secretion was delayed and concomitant with T cell activation. In an influenza-induced exacerbation of allergic airway inflammation model we noticed an initial reduction of ILC2 numbers and cytokine production in broncho-alveolar lavage compared to chronic house dust mite (HDM)-mediated airway inflammation alone. ILC2s phenotype was characterized by low T1/ST2, ICOS, KLRG1, and CD25 expression, resembling naïve ILC2s. The contribution of ILC2s to type 2 cytokine production in the early stage of the influenza-induced exacerbation was limited. In contrast, T cells showed increased IL-4 and IL-5 production when exposed to both HDM and influenza virus. Upon virus clearance, ILC2s regained an activated T1/ST2high ICOShigh KLRG1high CD25high phenotype paired with cytokine production and were major contributors to the type 2 cytokine milieu. Collectively, our data indicate that both T cells and ILC2s contribute to influenza-induced exacerbation of allergic airway inflammation, but with different kinetics.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Orthomyxoviridae
/
Respiratory System
/
Lymphocytes
/
Orthomyxoviridae Infections
/
Th2 Cells
/
GATA3 Transcription Factor
/
Influenza, Human
/
Hypersensitivity
/
Inflammation
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Eur J Immunol
Year:
2019
Document type:
Article
Affiliation country:
Netherlands