Signal sequence-independent targeting of MID2 mRNA to the endoplasmic reticulum by the yeast RNA-binding protein Khd1p.
FEBS Lett
; 592(11): 1870-1881, 2018 06.
Article
in En
| MEDLINE
| ID: mdl-29772604
ABSTRACT
Localization of mRNAs depends on specific RNA-binding proteins (RBPs) and critically contributes not only to cell polarization but also to basal cell function. The yeast RBP Khd1p binds to several hundred mRNAs, the majority of which encodes secreted or membrane proteins. We demonstrate that a subfraction of Khd1p associates with artificial liposomes and endoplasmic reticulum (ER), and that Khd1p endomembrane association is partially dependent on its binding to RNA. ER targeting of at least two mRNAs, MID2 and SLG1/WSC1, requires KHD1 but is independent of their translation. Together, our results suggest interdependence of Khd1p and mRNA for their targeting to the ER and presents additional evidence for signal sequence-independent, RBP-mediated mRNA targeting.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribonucleoproteins
/
Saccharomyces cerevisiae
/
RNA, Fungal
/
RNA, Messenger
/
Membrane Glycoproteins
/
Saccharomyces cerevisiae Proteins
/
Intracellular Signaling Peptides and Proteins
/
Endoplasmic Reticulum
Language:
En
Journal:
FEBS Lett
Year:
2018
Document type:
Article
Affiliation country:
Germany