A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells.
Metab Eng
; 48: 72-81, 2018 07.
Article
in En
| MEDLINE
| ID: mdl-29852271
Key words
BFP, Blue Fluorescent Protein; BP, Bandpass; CD, Chemically defined; CHO, Chinese Hamster ovary; CRISPR, Clustered regularly interspaced palindromic repeats; CRISPR/Cas9; Cas9, CRISPR-associated protein 9; Cell line engineering; Chinese Hamster; Fluorescent proteins; GFP, Green Fluorescent Protein; MAARGE, Multiplexable Activation of Artificially Repressed Genes; MFI, Mean fluorescence intensity; Ovary cells CHO; Pathway engineering; RE, Repressor element; REST, Repressor element 1 silencing transcription factor; RFP, Red Fluorescent protein; RFP657, Red Fluorescent protein 657; bp, Base pairs; gRNA, Guide RNA; poly(A), Poly Adenylation signal; rpm, Rotations per minute
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Engineering
/
Gene Expression
/
CRISPR-Cas Systems
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Metab Eng
Journal subject:
ENGENHARIA BIOMEDICA
/
METABOLISMO
Year:
2018
Document type:
Article
Affiliation country:
Austria
Country of publication:
Belgium