Your browser doesn't support javascript.
loading
Genotyping-by-sequencing of genome-wide microsatellite loci reveals fine-scale harvest composition in a coastal Atlantic salmon fishery.
Bradbury, Ian R; Wringe, Brendan F; Watson, Beth; Paterson, Ian; Horne, John; Beiko, Robert; Lehnert, Sarah J; Clément, Marie; Anderson, Eric C; Jeffery, Nicholas W; Duffy, Steven; Sylvester, Emma; Robertson, Martha; Bentzen, Paul.
Affiliation
  • Bradbury IR; Science Branch Department of Fisheries and Oceans Canada St. John's NL Canada.
  • Wringe BF; Department of Biology Dalhousie University Halifax NS Canada.
  • Watson B; Faculty of Computer Science Dalhousie University Halifax NS Canada.
  • Paterson I; Science Branch Department of Fisheries and Oceans Canada St. John's NL Canada.
  • Horne J; Department of Biology Dalhousie University Halifax NS Canada.
  • Beiko R; Department of Biology Dalhousie University Halifax NS Canada.
  • Lehnert SJ; Department of Biology Dalhousie University Halifax NS Canada.
  • Clément M; Faculty of Computer Science Dalhousie University Halifax NS Canada.
  • Anderson EC; Science Branch Department of Fisheries and Oceans Canada St. John's NL Canada.
  • Jeffery NW; Center for Fisheries Ecosystems Research Fisheries and Marine Institute of Memorial University of Newfoundland St. John's NL Canada.
  • Duffy S; Labrador Institute of Memorial University of Newfoundland Happy Valley-Goose Bay NL Canada.
  • Sylvester E; Fisheries Ecology Division Southwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration Santa Cruz CA USA.
  • Robertson M; Science Branch Department of Fisheries and Oceans Canada St. John's NL Canada.
  • Bentzen P; Science Branch Department of Fisheries and Oceans Canada St. John's NL Canada.
Evol Appl ; 11(6): 918-930, 2018 Jul.
Article in En | MEDLINE | ID: mdl-29928300
ABSTRACT
Individual assignment and genetic mixture analysis are commonly utilized in contemporary wildlife and fisheries management. Although microsatellite loci provide unparalleled numbers of alleles per locus, their use in assignment applications is increasingly limited. However, next-generation sequencing, in conjunction with novel bioinformatic tools, allows large numbers of microsatellite loci to be simultaneously genotyped, presenting new opportunities for individual assignment and genetic mixture analysis. Here, we scanned the published Atlantic salmon genome to identify 706 microsatellite loci, from which we developed a final panel of 101 microsatellites distributed across the genome (average 3.4 loci per chromosome). Using samples from 35 Atlantic salmon populations (n = 1,485 individuals) from coastal Labrador, Canada, a region characterized by low levels of differentiation in this species, this panel identified 844 alleles (average of 8.4 alleles per locus). Simulation-based evaluations of assignment and mixture identification accuracy revealed unprecedented resolution, clearly identifying 26 rivers or groups of rivers spanning 500 km of coastline. This baseline was used to examine the stock composition of 696 individuals harvested in the Labrador Atlantic salmon fishery and revealed that coastal fisheries largely targeted regional groups (<300 km). This work suggests that the development and application of large sequenced microsatellite panels presents great potential for stock resolution in Atlantic salmon and more broadly in other exploited anadromous and marine species.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Evol Appl Year: 2018 Document type: Article Publication country: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Evol Appl Year: 2018 Document type: Article Publication country: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM