Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis.
Exp Cell Res
; 370(2): 273-282, 2018 09 15.
Article
in En
| MEDLINE
| ID: mdl-29953877
ABSTRACT
Nonmuscle myosin-IIA (NMHC-IIA) heavy chain phosphorylation has gained recognition as an important feature of myosin-II regulation. In previous work, we showed that phosphorylation on S1943 promotes myosin-IIA filament disassembly in vitro and enhances EGF-stimulated lamellipod extension of breast tumor cells. However, the contribution of NMHC-IIA S1943 phosphorylation to the modulation of invasive cellular behavior and metastasis has not been examined. Stable expression of phosphomimetic (S1943E) or non-phosphorylatable (S1943A) NMHC-IIA in breast cancer cells revealed that S1943 phosphorylation enhances invadopodia function, and is critical for matrix degradation in vitro and experimental metastasis in vivo. These studies demonstrate a novel link between NMHC-IIA S1943 phosphorylation, the regulation of extracellular matrix degradation and tumor cell invasion and metastasis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nonmuscle Myosin Type IIA
/
Cytoskeletal Proteins
/
Podosomes
/
Neoplasm Metastasis
Limits:
Humans
Language:
En
Journal:
Exp Cell Res
Year:
2018
Document type:
Article
Affiliation country:
United States