Regional differences in dopamine release in the R6/2 mouse caudate putamen.
Electroanalysis
; 30(6): 1066-1072, 2018 Jun.
Article
in En
| MEDLINE
| ID: mdl-29955208
ABSTRACT
Huntington's disease (HD) is a fatal neurodegenerative disorder that is characterized by degeneration of the striatum. Here, fast-scan cyclic voltammetry at carbon-fiber microelectrodes was used to uncover regional differences in dopamine (DA) release in the caudate putamen of R6/2 and wild-type control mice. We found a decreasing ventral-to-dorsal gradient in DA release, evoked by a single electrical stimulus pulse, in aged R6/2 mice. Moreover, under more intense stimulation conditions (120 pulses), DA release was significantly attenuated in the dorsal, but not in the ventral caudate. Autoradiography measurements using [3H]WIN 35,428 revealed that the overall density of DA transporter (DAT) protein molecules was significantly less in R6/2 mice compared to WT control mice; however, quadrants of the caudate putamen were not differentially altered in the R6/2 mice. These data collectively suggest that DA release in the dorsal caudate region is more vulnerable with age progression compared to the ventral region.
Full text:
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Electroanalysis
Year:
2018
Document type:
Article