Your browser doesn't support javascript.
loading
Three dimensional characterisation of chromatography bead internal structure using X-ray computed tomography and focused ion beam microscopy.
Johnson, T F; Bailey, J J; Iacoviello, F; Welsh, J H; Levison, P R; Shearing, P R; Bracewell, D G.
Affiliation
  • Johnson TF; Department of Biochemical Engineering, University College London, Bernard Katz, London, WC1E 6BT, United Kingdom.
  • Bailey JJ; Electrochemical Innovation Lab, Department of Chemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom.
  • Iacoviello F; Electrochemical Innovation Lab, Department of Chemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom.
  • Welsh JH; Pall Biotech, 5 Harbourgate Business Park, Southampton Road, Portsmouth, PO6 4BQ United Kingdom.
  • Levison PR; Pall Biotech, 5 Harbourgate Business Park, Southampton Road, Portsmouth, PO6 4BQ United Kingdom.
  • Shearing PR; Electrochemical Innovation Lab, Department of Chemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom.
  • Bracewell DG; Department of Biochemical Engineering, University College London, Bernard Katz, London, WC1E 6BT, United Kingdom. Electronic address: d.bracewell@ucl.ac.uk.
J Chromatogr A ; 1566: 79-88, 2018 Sep 07.
Article in En | MEDLINE | ID: mdl-29970222
X-ray computed tomography (CT) and focused ion beam (FIB) microscopy were used to generate three dimensional representations of chromatography beads for quantitative analysis of important physical characteristics including tortuosity factor. Critical-point dried agarose, cellulose and ceramic beads were examined using both methods before digital reconstruction and geometry based analysis for comparison between techniques and materials examined. X-ray 'nano' CT attained a pixel size of 63 nm and 32 nm for respective large field of view and high resolution modes. FIB improved upon this to a 15 nm pixel size for the more rigid ceramic beads but required compromises for the softer agarose and cellulose materials, especially during physical sectioning that was not required for X-ray CT. Digital processing of raw slices was performed using software to produce 3D representations of bead geometry. Porosity, tortuosity factor, surface area to volume ratio and pore diameter were evaluated for each technique and material, with overall averaged simulated tortuosity factors of 1.36, 1.37 and 1.51 for agarose, cellulose and ceramic volumes respectively. Results were compared to existing literature values acquired using established imaging and non-imaging techniques to demonstrate the capability of tomographic approaches used here.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tomography, X-Ray Computed / Microscopy Language: En Journal: J Chromatogr A Year: 2018 Document type: Article Affiliation country: United kingdom Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tomography, X-Ray Computed / Microscopy Language: En Journal: J Chromatogr A Year: 2018 Document type: Article Affiliation country: United kingdom Country of publication: Netherlands