Genome-wide C-SWAT library for high-throughput yeast genome tagging.
Nat Methods
; 15(8): 598-600, 2018 08.
Article
in En
| MEDLINE
| ID: mdl-29988096
ABSTRACT
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Genomic Library
/
Genome, Fungal
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2018
Document type:
Article
Affiliation country:
Germany