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Structural differences between toxic and nontoxic HypF-N oligomers.
Capitini, Claudia; Patel, Jayneil R; Natalello, Antonino; D'Andrea, Cristiano; Relini, Annalisa; Jarvis, James A; Birolo, Leila; Peduzzo, Alessia; Vendruscolo, Michele; Matteini, Paolo; Dobson, Christopher M; De Simone, Alfonso; Chiti, Fabrizio.
Affiliation
  • Capitini C; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Firenze, Italy. fabrizio.chiti@unifi.it.
  • Patel JR; Department of Life Sciences, Imperial College London, South Kensington, SW72AZ London, UK.
  • Natalello A; Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
  • D'Andrea C; Institute of Applied Physics, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
  • Relini A; Department of Chemistry and Industrial Chemistry, University of Genoa, Via Dodecaneso 31, 16146, Genova, Italy.
  • Jarvis JA; Department of Life Sciences, Imperial College London, South Kensington, SW72AZ London, UK.
  • Birolo L; Department of Chemical Sciences, University of Naples "Federico II", Monte S. Angelo Campus, Via Cinthia, 80126, Napoli, Italy.
  • Peduzzo A; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Firenze, Italy. fabrizio.chiti@unifi.it.
  • Vendruscolo M; Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
  • Matteini P; Institute of Applied Physics, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
  • Dobson CM; Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
  • De Simone A; Department of Life Sciences, Imperial College London, South Kensington, SW72AZ London, UK.
  • Chiti F; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Firenze, Italy. fabrizio.chiti@unifi.it.
Chem Commun (Camb) ; 54(62): 8637-8640, 2018 Aug 11.
Article in En | MEDLINE | ID: mdl-30020284
ABSTRACT
We have studied two misfolded oligomeric forms of the protein HypF-N, which show similar morphologies but very different toxicities. We measured over 80 intermolecular distance-dependent parameters for each oligomer type using FRET, in conjunction with solution- and solid-state NMR and other biophysical techniques. The results indicate that the formation of a highly organised hydrogen bonded core in the toxic oligomers results in the exposure of a larger number of hydrophobic residues than in the nontoxic species, causing the former to form aberrant interactions with cellular components.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carboxyl and Carbamoyl Transferases / Escherichia coli Proteins Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2018 Document type: Article Affiliation country: Italy
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carboxyl and Carbamoyl Transferases / Escherichia coli Proteins Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2018 Document type: Article Affiliation country: Italy