Determination of gentamicin C components in fish tissues through SPE-Hypercarb-HPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1093-1094: 167-173, 2018 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-30031305
ABSTRACT
In this work, an HPLC/MS/MS method for determination of gentamicin C components in fish tissues was developed based on strong cation exchange solid-phase extraction (SPE) purification coupled with Hypercarb chromatographic column in separation mode. Sample was extracted using trichloroacetic acid aqueous solution containing EDTA. Ion-pairing reagents were not needed because of the "graphite polarity retention effect" of the Hypercarb chromatographic column. HPLC-MS/MS was performed in multiple reaction monitoring (MRM) mode for simultaneous qualitative and quantitative analyses (using matrix external standard) of gentamicin C components in fish tissues. Good linearity was obtained for the target analytes within the concentration range from 0.0100 to 0.500â¯mg/L. The limits of quantification (LOQ) of this method were 10.0, 20.0, and 20.0⯵g/kg for C1, C1a, and sum of C2â¯+â¯C2a, respectively. The average recoveries of gentamicin C components were 80.0%-110% when spiked at three levels with the blank carp (Cyprinus carpio) matrix, and the relative standard deviations (RSD) were all less than 15% (nâ¯=â¯6). In addition, for the features of simple operation, high sensitivity and good reproducibility, the proposed method has been successfully applied for detection of gentamicin residues in fish tissues during actual breeding.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carps
/
Drug Residues
/
Gentamicins
/
Chromatography, High Pressure Liquid
/
Meat
Type of study:
Prognostic_studies
/
Qualitative_research
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2018
Document type:
Article