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Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy.
Das, Manabendra; Du, Yang; Mortensen, Jonas S; Hariharan, Parameswaran; Lee, Hyun Sung; Byrne, Bernadette; Loland, Claus J; Guan, Lan; Kobilka, Brian K; Chae, Pil Seok.
Affiliation
  • Das M; Department of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Ansan, 155-88, Korea.
  • Du Y; Present address: Molecular Biophysics, Technische Universität Kaiserslautern, Erwin-Schrödinger-Strasse 13, 67663, Kaiserslautern, Germany.
  • Mortensen JS; Molecular and Cellular Physiology, Stanford University, 279 Campus Drive, Stanford, CA, 94305, USA.
  • Hariharan P; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen, Denmark.
  • Lee HS; Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4th St. MS 6551, Lubbock, TX, 79430, USA.
  • Byrne B; Department of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Ansan, 155-88, Korea.
  • Loland CJ; Department of Life Sciences, Imperial College London, Exhibition Road, London, SW7 2AZ, UK.
  • Guan L; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen, Denmark.
  • Kobilka BK; Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4th St. MS 6551, Lubbock, TX, 79430, USA.
  • Chae PS; Molecular and Cellular Physiology, Stanford University, 279 Campus Drive, Stanford, CA, 94305, USA.
Chembiochem ; 19(20): 2225-2232, 2018 10 18.
Article in En | MEDLINE | ID: mdl-30070754
A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750-16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Receptors, Adrenergic, beta-2 / Amino Acid Transport Systems / Symporters / Detergents / Membrane Proteins Limits: Humans Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2018 Document type: Article Country of publication: Germany
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Receptors, Adrenergic, beta-2 / Amino Acid Transport Systems / Symporters / Detergents / Membrane Proteins Limits: Humans Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2018 Document type: Article Country of publication: Germany