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A Green BODIPY-Based, Super-Fluorogenic, Protein-Specific Labelling Agent.
Chen, Yingche; Tsao, Kelvin; Acton, Sydney L; Keillor, Jeffrey W.
Affiliation
  • Chen Y; Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.
  • Tsao K; Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.
  • Acton SL; Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.
  • Keillor JW; Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.
Angew Chem Int Ed Engl ; 57(38): 12390-12394, 2018 09 17.
Article in En | MEDLINE | ID: mdl-30086191
ABSTRACT
We report the development of YC23, a novel green BODIPY-based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Boron Compounds / Fluorescent Dyes Limits: Humans Language: En Journal: Angew Chem Int Ed Engl Year: 2018 Document type: Article Affiliation country: Canada
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Boron Compounds / Fluorescent Dyes Limits: Humans Language: En Journal: Angew Chem Int Ed Engl Year: 2018 Document type: Article Affiliation country: Canada