[Cloning,Bioinformatic Analysis of Chalcone-Flavonone Isomerase Gene( CHI) and Relationship Between Expression of CHI and Accumulation of HSYA in Carthamus tinctorius].
Zhong Yao Cai
; 39(3): 499-503, 2016 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-30088872
ABSTRACT
Objective:
To clone chalcone-flavonone isomerase( CHI) gene in Carthamus tinctorius,to analyze the bioinformation of CHI,to compare the expression of CHI,and to analyze the percentage composition of hydroxysafflor yellow A( HSYA) during the florescence,in order to provide the foundation for functional verification of CHI and the composition and regulation mechanism of the flavonoid constituents in Carthamus tinctorius.Methods:
CHI was cloned,bioinformatics was used to analyze the protein characteristics, real timePCR was used to analyze the expression of CHI,and HPLC was used to analyze the percentage composition of HSYAResults:
A 696 bp CHI sequence in Carthamus tinctorius,the expression of CHI and the percentage composition of HSYA during the florescence were obtained. The variation tendency was similar between the expression of CHI and the accumulation of HSYA, which was increased gradually during 1 ~ 4 d and peaked at the fourth day,then decreased sharply during the fifth to the seventh day of florescence.Conclusion:
This research provides a foundation for functional verification of CHI and the composition and regulation mechanism of the flavonoid constituents in Carthamus tinctorius.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carthamus tinctorius
Language:
Zh
Journal:
Zhong Yao Cai
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2016
Document type:
Article