Plasmonic meta-electrodes allow intracellular recordings at network level on high-density CMOS-multi-electrode arrays.
Nat Nanotechnol
; 13(10): 965-971, 2018 10.
Article
in En
| MEDLINE
| ID: mdl-30104618
ABSTRACT
The ability to monitor electrogenic cells accurately plays a pivotal role in neuroscience, cardiology and cell biology. Despite pioneering research and long-lasting efforts, the existing methods for intracellular recording of action potentials on the large network scale suffer limitations that prevent their widespread use. Here, we introduce the concept of a meta-electrode, a planar porous electrode that mimics the optical and biological behaviour of three-dimensional plasmonic antennas but also preserves the ability to work as an electrode. Its synergistic combination with plasmonic optoacoustic poration allows commercial complementary metal-oxide semiconductor multi-electrode arrays to record intracellular action potentials in large cellular networks. We apply this approach to measure signals from human-induced pluripotent stem cell-derived cardiac cells, rodent primary cardiomyocytes and immortalized cell types and demonstrate the possibility of non-invasively testing a variety of relevant drugs. Due to its robustness and easiness of use, we expect the method will be rapidly adopted by the scientific community and by pharmaceutical companies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semiconductors
/
Action Potentials
/
Myocytes, Cardiac
/
Photoacoustic Techniques
Limits:
Animals
/
Humans
Language:
En
Journal:
Nat Nanotechnol
Year:
2018
Document type:
Article
Affiliation country:
Italy