Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene.
Beilstein J Org Chem
; 14: 1840-1845, 2018.
Article
in En
| MEDLINE
| ID: mdl-30112088
ABSTRACT
Based on the indicator displacement assay (IDA) approach, we herein report the fluorescence "switch-on" sensing and quantitative detection of bisphosphonates (BPs), a class of drugs extensively used in the treatment of patients with various skeletal diseases. Guanidinium-modified calix[5]arene (GC5A) affords strong binding on the micromolar to nanomolar level towards BPs dominantly via multiple salt bridge interactions, which was evaluated by fluorescence competitive titrations. Fluorescent IDA enables the highly sensitive and label-free detection of BPs in buffer solution, and more importantly, in artificial urine. Calibration lines were therefore set up in untreated artificial urine, allowing for quantifying the concentrations of BPs in the biologically relevant low range.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Beilstein J Org Chem
Year:
2018
Document type:
Article
Affiliation country:
China