A mini-STR typing system for degraded equine DNA.
Anim Genet
; 49(5): 464-466, 2018 Oct.
Article
in En
| MEDLINE
| ID: mdl-30117168
ABSTRACT
Degraded biological samples are a challenge for testing laboratories. Genotyping success can be improved through the use of mini-STRs, by which primers are placed adjacent to the repeat motifs to reduce amplicon size. Here, we present a genetic profiling system comprising 13 autosomal and one X-linked dinucleotide-repeat markers and the SRY gene based on the internationally accepted equine parentage panel. The markers are divided into two panels with all alleles falling at or below 182 bp. The application of this method significantly increases the ability to profile difficult samples and to provide discriminating results to clients.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sequence Analysis, DNA
Limits:
Animals
Language:
En
Journal:
Anim Genet
Journal subject:
GENETICA
/
MEDICINA VETERINARIA
Year:
2018
Document type:
Article
Affiliation country:
United States