Clinical usefulness of multiplex PCR-lateral flow for the diagnosis of orthopedic-related infections.
Mod Rheumatol
; 29(5): 867-873, 2019 Sep.
Article
in En
| MEDLINE
| ID: mdl-30126317
ABSTRACT
Objectives:
Polymerase chain reaction (PCR)-based assays are being increasingly used for the diagnosis of orthopedic-related infections. Unfortunately, classical PCR requires imaging devices that are expensive and complex. We previously developed the PCR-lateral flow (PCR-LF) method, which does not require any additional imaging device. In the present study, the objective was to determine whether PCR-LF tests could be used to effectively diagnose orthopedic-related infections.Methods:
In this study, we used PCR-LF to diagnose common causes of orthopedic-related infections and compared the results to those from conventional bacterial cultures of the same samples.Results:
Notably, for 228 synovial fluid or pus specimens, the sensitivity and specificity of bacterial cultures were 53.5% and 97.7%, respectively, compared to 61.6% and 89.9% for PCR-LF. Although the difference in sensitivity between bacterial cultures and PCR-LF was not significant, when our analysis was limited to cases with suspected periprosthetic joint infection, the sensitivity of PCR-LF (66.1%) was superior to that of bacterial cultures (42.9%).Conclusion:
This study indicates that PCR-LF is a useful method for diagnosing orthopedic-related infections.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arthritis, Infectious
/
Prosthesis-Related Infections
/
Molecular Diagnostic Techniques
/
Multiplex Polymerase Chain Reaction
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Mod Rheumatol
Year:
2019
Document type:
Article
Affiliation country:
Japan