Microscope calibration using laser written fluorescence.
Opt Express
; 26(17): 21887-21899, 2018 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-30130891
ABSTRACT
There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2018
Document type:
Article