Bone marrow-derived stem/stromal cells (BMSC) 3D microtissues cultured in BMP-2 supplemented osteogenic induction medium are prone to adipogenesis.
Cell Tissue Res
; 374(3): 541-553, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30136155
ABSTRACT
Bone marrow-derived mesenchymal stem/stromal cells (BMSC) may facilitate bone repair through secretion of factors that stimulate endogenous repair processes or through direct contribution to new bone through differentiation into osteoblast-like cells. BMSC microtissue culture and differentiation has been widely explored recently, with high-throughput platforms making large-scale manufacture of microtissues increasingly feasible. Bone-like BMSC microtissues could offer an elegant method to enhance bone repair, especially in small-volume non-union defects, where small diameter microtissues could be delivered orthoscopically. Using a high-throughput microwell platform, our data demonstrate that (1) BMSC in 3D microtissue culture result in tissue compaction, rather than growth, (2) not all mineralised bone-like matrix is incorporated in the bulk microtissue mass and (3) a significant amount of lipid vacuole formation is observed in BMSC microtissues exposed to BMP-2. These factors should be considered when optimising BMSC osteogenesis in microtissues or developing BMSC microtissue-based therapeutic delivery processes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Culture Media
/
Tissue Engineering
/
Tissue Culture Techniques
/
Adipogenesis
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Bone Morphogenetic Protein 2
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Mesenchymal Stem Cells
Limits:
Adult
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Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Cell Tissue Res
Year:
2018
Document type:
Article
Affiliation country:
Australia