Effect of selenomethionine on cell viability and heat shock protein 70 levels in rainbow trout intestinal epithelial cells at hypo-, normo-, and hyper-thermic temperatures.
J Therm Biol
; 76: 107-114, 2018 Aug.
Article
in En
| MEDLINE
| ID: mdl-30143285
ABSTRACT
As global warming and environmental pollution modify aquatic environments, the thermal biology of fish could be affected by interactions between temperature and pollutants, such as selenium (Se). Therefore, selenomethionine (SeMet) was studied for effects on cell viability and on heat shock protein 70 (HSP70) levels in the rainbow trout intestinal epithelial cell, RTgutGC, at hypothermic (4⯰C), normothermic (14 and 18⯰C) and hyperthermic (26⯰C) temperatures. RTgutGC cultures remained viable for at least a week at all temperatures, although energy metabolism as measured with Alamar Blue (resazurin) was appreciably diminished at 4⯰C. Over a 7-day incubation, HSP 70 levels in cultures remained steady at 4⯰C, declined at 18⯰C, and increased slightly at 26⯰C. When 125⯵M SeMet was present, cultures remained viable and HSP70 levels were neither increased nor decreased relative to control cultures, regardless of the temperature. With 500 and 1000⯵M SeMet, cell viability was profoundly impaired after 7 days in cultures at 14, 18 and 26⯰C but was unchanged at 4⯰C. Overall the results suggest that only hypothermia modulated the response of rainbow trout cells to SeMet.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Selenomethionine
/
HSP70 Heat-Shock Proteins
/
Epithelial Cells
Limits:
Animals
Language:
En
Journal:
J Therm Biol
Year:
2018
Document type:
Article