Comparative analysis of stimulation and binding characteristics of adenosine analogs to AMP-activated protein kinase.
J Asian Nat Prod Res
; 21(9): 916-927, 2019 Sep.
Article
in En
| MEDLINE
| ID: mdl-30187782
ABSTRACT
To compare the stimulation and binding characteristics of adenosine analogs including AMP, IMM-H007, and M1, to AMPK, and to explore the potential mechanism underlying the regulation effect of adenosine analogs on AMPK activity, [γ-32P]ATP assay, circular dichroism experiments and molecular docking test were performed. We found that the interactions with Thr86, Thr88, and His150 in site 1 are probably the reason why the affinities of IMM-H007, M1, and adenosine are comparable but their allosteric activation on AMPK varies greatly, partly interpreting the mechanism of AMPK activity regulated by adenosine analogs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenosine
/
AMP-Activated Protein Kinases
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Asian Nat Prod Res
Journal subject:
BOTANICA
/
QUIMICA
Year:
2019
Document type:
Article
Affiliation country:
China