Cloning, characterization and function analysis of DAX1 in Chinese loach (Paramisgurnus dabryanus).
Genetica
; 146(6): 487-496, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30206752
ABSTRACT
The mechanisms of sex determination and differentiation have not been elucidated in most fish species. In this study, the full-length cDNAs of DAX1 was cloned and characterized in aquaculture fish Chinese loach (Paramisgurnus dabryanus), designated as Pd-DAX1. The cDNA sequence of Pd-DAX1 was 1261 bp, including 795 bp open reading frame (ORF) encoding 264 amino acids. Pd-DAX1 shares highly identical sequence with DAX1 homologues from different species. The expression profiles of Pd-DAX1 in different developmental stages and diverse adult tissues were analyzed by quantitative real-time RT-PCR and in situ hybridization (ISH). Pd-DAX1 was continuously expressed during embryogenesis, with the extensive distribution in the development of the central nervous system. Tissue distribution analysis revealed that Pd-DAX1 expressed widely in adult tissues, with the highest expression level found in testis, moderate level in ovary, showing a sex-dimorphic expression pattern. Pd-DAX1 mainly located in spermatogonia cells, spermatocytes, primary oocytes and previtellogenic oocyte cells, implying that Pd-DAX1 may involve in gametogenesis. These preliminary findings suggest that Pd-DAX1 gene is highly conserved during vertebrate evolution and involved in a wide range of developmental processes including embryogenesis, central nervous system development and gonad development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cypriniformes
/
Fish Proteins
/
DAX-1 Orphan Nuclear Receptor
Limits:
Animals
Language:
En
Journal:
Genetica
Year:
2018
Document type:
Article