Polymicrobial antibiofilm activity of the membranotropic peptide gH625 and its analogue.
Microb Pathog
; 125: 189-195, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30227230
ABSTRACT
This work illustrates a new role for the membranotropic peptide gH625 and its derivative gH625-GCGKKK in impairing formation of polymicrobial biofilms. Mixed biofilms composed of Candida and bacterial species cause frequently infections and failure of medical silicone devices and also show a major drug resistance than single-species biofilms. Inhibition and eradication of biofilms were evaluated by complementary methods:
XTT-reduction, and crystal violet staining (CV). Our results indicate that gH625-GCGKKKK, better than the native peptide, strongly inhibited formation of mixed biofilms of clinical isolates of C. tropicalis/S. marcescens and C. tropicalis/S. aureus and reduced the biofilm architecture, interfering with cell adhesion and polymeric matrix, as well as eradicated the long-term polymicrobial biofilms on silicone surface.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Serratia marcescens
/
Staphylococcus aureus
/
Viral Envelope Proteins
/
Biofilms
/
Candida tropicalis
/
Anti-Infective Agents
Language:
En
Journal:
Microb Pathog
Journal subject:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
Italy