Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.
PLoS One
; 13(9): e0204677, 2018.
Article
in En
| MEDLINE
| ID: mdl-30256839
ABSTRACT
Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 61 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coculture Techniques
/
Tissue Engineering
/
Myocytes, Smooth Muscle
/
Endothelial Progenitor Cells
Type of study:
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
PLoS One
Journal subject:
CIENCIA
/
MEDICINA
Year:
2018
Document type:
Article
Affiliation country:
China