Categorical performance characteristics of method ISO 7899-2 and indicator value of intestinal enterococci for bathing water quality monitoring.
J Water Health
; 16(5): 711-723, 2018 Oct.
Article
in En
| MEDLINE
| ID: mdl-30285953
ABSTRACT
Intestinal enterococci indicate the fecal contamination of bathing waters. This study defines the performance characteristics of the reference method ISO 7899-22000 with water samples collected from inland and coastal bathing areas in Finland. From a total of 341 bacterial isolates grown on Slanetz and Bartley medium, 63.6% were confirmed as intestinal enterococci on bile aesculin agar. The partial 16S rRNA gene sequences showed that Enterococcus faecium and Enterococcus faecalis clades accounted for 93.1% of the confirmed isolates. The range of the false positive and false negative rate of the ISO 7899-2 was 0.0-18.5% and 5.6-57.1%, respectively, being affected by the presumptive colony count on the membrane. The analysis of multiple sample volumes is proposed to reach 10-100 colonies per membrane when 47 mm diameter membranes are used to prevent overestimation of low counts and underestimation of the high counts.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Water Microbiology
/
Environmental Monitoring
/
Enterococcus
Country/Region as subject:
Europa
Language:
En
Journal:
J Water Health
Journal subject:
SAUDE AMBIENTAL
Year:
2018
Document type:
Article