Efficacy of using erbium, chromium-doped: Yttrium scandium gallium garnet laser-treated dentine in a dentine barrier test device.

ABSTRACT

OBJECTIVES: The aim of this study was to evaluate the efficacy of using erbium, chromium-dopedyttrium scandium gallium garnet (Er,CrYSGG) laser-treated dentine in a dentine barrier test device. MATERIALS AND METHODS: The test materials (G-Bond™ and Vitrebond™) were applied onto laser-treated or laser-untreated dentine discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated based on enzyme activity and compared to a nontoxic control material. The mean of the control was set to 100% viability. Data were analyzed using the one-way analysis of variance and the Tukey's honest significant difference tests. RESULTS: The responses of bovine pulp-derived cells after exposure to G-Bond and Vitrebond on Er,CrYSGG laser-treated and laser-untreated dentin were statistically different from negative control group (P < 0.05). CONCLUSION: Er,CrYSGG laser treatment was not successful enough in decreasing the cytotoxic effects of the dental materials. Different parameters of Er,CrYSGG laser or different laser types could be investigated as an alternative to minimizing the cytotoxic effects of dental materials.