Digital PCR detection of plasmid DNA administered to the skeletal muscle of a microminipig: a model case study for gene doping detection.
BMC Res Notes
; 11(1): 708, 2018 Oct 10.
Article
in En
| MEDLINE
| ID: mdl-30309394
ABSTRACT
OBJECTIVE:
Doping control is an important and indispensable aspect of fair horse racing; genetic doping has been recently included to this. In this study, we aimed to develop a detection method of gene doping. A plasmid cloned with human erythropoietin gene (p.hEPO, 250 µg/head) was intramuscularly injected into a microminipig. Subsequently, p.hEPO was extracted from 1 mL of plasma and detected by droplet digital polymerase chain reaction.RESULTS:
The results confirmed that the maximum amount of plasmid was detected at 15 min after administration and the majority of the plasmid was degraded in the bloodstream within 1-2 days after administration. In contrast, low amounts of p.hEPO were detected at 2-3 weeks after administration. These results suggest that the proposed method to detect gene doping can help obtain information for experiments using horses.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Polymerase Chain Reaction
/
Erythropoietin
/
Doping in Sports
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
BMC Res Notes
Year:
2018
Document type:
Article
Affiliation country:
Japan