NRF2 and HSF1 coordinately regulate heme oxygenase-1 expression.
Biochem Biophys Res Commun
; 506(1): 7-11, 2018 11 17.
Article
in En
| MEDLINE
| ID: mdl-30316516
ABSTRACT
Heme oxygenase-1 (HO-1) is an inducible enzyme responding to various stresses and has cytoprotective activities. Although HO-1 has been referred to as heat shock protein (HSP) 32, the heat-mediated induction of HO-1 varies among different species and cell lines. We examined the effects of heat shock on HO-1 expression in mouse embryonic fibroblast (MEF) cells deficient in heat shock factor 1 (HSF1) or nuclear factor-erythroid-2-related factor 2 (NRF2). Heme-induced expression of HO-1 was 2-fold higher in Hsf1-/- cells than in the wild-type cells at both mRNA and protein levels. In Nrf2-/- cells, heme-induced expression of HO-1 was not detected. In contrast, HO-1 expression was markedly induced by heat shock at 40-42⯰C in Nrf2-/- cells while the wild-type cells were not responsive. The heat-induced expression of HO-1 in Nrf2-/- cells were almost completely diminished by transfection of siRNA against Hsf1 gene. These results suggest that HSF1 and NRF2 suppress heme-induced and heat-induced HO-1 expression, respectively.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Heat-Shock Response
/
Heme Oxygenase-1
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NF-E2-Related Factor 2
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Fibroblasts
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Heat Shock Transcription Factors
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Membrane Proteins
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2018
Document type:
Article