Association between the PPARa and PPARGCA gene variations and physical performance in non-trained male adolescents.

ABSTRACT

The purpose of the research was to examine if some genetic variations are associated with some endurance, power and speed performances (multi-stage 20-m shuttle run, standing broad jump, 20 m sprint test and Abalakov jump) in a group of 586 non-trained male adolescents (mean ± SD age 13.20 ± 0.25 years). Polymorphisms in PPARa and PPARGC1A implicated in physical performance traits were analyzed. DNA was extracted and the samples were genotyped for PPARa and PPARGC1A polymorphisms by a PCR based method followed by gel electrophoresis. The discrepancies in the study phenotypes among variations of the PPARa and PPARGC1A polymorphisms were analyzed by one-way analysis of covariance (ANCOVA), after age, weight and height adjustment. To examine whether the genotype and allele frequencies between adolescents with high and low performances were different, we divided them into two groups ≥ 90th and < 90th of the percentile. The genotype and allele frequencies between adolescents with high and low performances were compared with the Chi square test. Our analysis demonstrated the effects of the PPARa and PPARGC1A polymorphisms only on [Formula see text] (p = 0.010 and p = 0.010 respectively). Also, we observed significant differences in PPARa and PPARGC1A genotypes (p = 0.034 and p = 0.024) or allele frequencies (p = 0.031 and p = 0.001) between groups for the multi-stage 20-m shuttle run test. Findings of this research suggest that both the PPARa and PPARGC1A polymorphisms are associated with estimating endurance-related phenotype and endurance capacity in male non-athletes adolescents.