Separation of blood group A-active oligosaccharides by high-pressure liquid affinity chromatography using a monoclonal antibody bound to concanavalin A silica.
Anal Biochem
; 161(1): 140-3, 1987 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-3034096
ABSTRACT
A column for high-pressure liquid affinity chromatography is prepared by binding a murine monoclonal anti-blood group A antibody of IgM isotype to concanavalin A-coated silica particles. The column specifically retards blood group A-active oligosaccharides with the nonreducing immunodominant trisaccharide sequence, GalNAc alpha 1-3(Fuc alpha 1-2)Gal beta 1- ..., and separates three A-active oligosaccharides with different core structures. Retention of the oligosaccharides on the column diminishes with increasing temperatures, permitting thermal elution in the range 25-50 degrees C.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligosaccharides
/
ABO Blood-Group System
/
Chromatography, Affinity
Limits:
Humans
Language:
En
Journal:
Anal Biochem
Year:
1987
Document type:
Article
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA