Coumarin Triazabutadienes for Fluorescent Labeling of Proteins.
Chembiochem
; 19(24): 2550-2552, 2018 12 18.
Article
in En
| MEDLINE
| ID: mdl-30341988
ABSTRACT
The use of small-molecule fluorophores to label proteins with minimal perturbation in response to an external stimulus is a powerful tool to probe chemical and biochemical environments. Herein, we describe the use of a coumarin-modified triazabutadiene that can deliver aryl diazonium ions to fluorescently label proteins by tyrosine-selective modification. The labeling can be triggered by low-pH-induced liberation of the diazonium species, thus making the fluorophore especially useful in labeling biochemical surroundings such as those found within the late endosome. Additionally, we show that a variety of coumarin triazabutadienes might also be prone to releasing their diazonium cargo after irradiation with UV light.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Triazenes
/
Molecular Probes
/
Proteins
/
Coumarins
/
Fluorescent Dyes
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2018
Document type:
Article
Affiliation country:
United States
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY