Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells.
Cancer Genomics Proteomics
; 15(6): 473-483, 2018.
Article
in En
| MEDLINE
| ID: mdl-30343281
ABSTRACT
BACKGROUND/AIM:
Resistance to anoikis is a pre-requisite step in metastasis, a major cause of death in patients with cancer, including thyroid cancer. Impairing anoikis resistance is a possible strategy for therapy of metastatic cancer. We, therefore, we aimed to investigate the key players of anoikis resistance. MATERIALS ANDMETHODS:
Papillary-type (BCPAP), follicular-type (FTC133), and anaplastic-type (ARO) thyroid carcinoma cells, cultured in poly(2-hydroxyethyl methacrylate)-coated plates to mimic circulating cells, were used as model systems in this study. Flow cytometry and soft-agar assays were used to determine cells exhibiting anoikis resistance. Proteomics was used to identify candidate proteins and validated using western blot and siRNA knockdown.RESULTS:
Only ARO cells showed both anoikis resistance potential and anchorage-independent growth ability. Tumor susceptibility gene 101 protein (TSG101) was identified to be potentially important in anoikis resistance, which was confirmed by an increase in anoikis and expression of a pro-apoptotic protein (BCL-2 like protein 4) and an apoptotic marker (cleaved poly-ADP ribose polymerase) in floating siTSG101-knockdown cells.CONCLUSION:
To our knowledge, this is the first study that implicates the importance of TSG101 in anoikis resistance of thyroid cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Thyroid Neoplasms
/
Anoikis
/
DNA-Binding Proteins
/
Endosomal Sorting Complexes Required for Transport
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Cancer Genomics Proteomics
Journal subject:
BIOQUIMICA
/
GENETICA MEDICA
/
NEOPLASIAS
Year:
2018
Document type:
Article
Affiliation country:
Thailand