NOTUM Is Involved in the Progression of Colorectal Cancer.
Cancer Genomics Proteomics
; 15(6): 485-497, 2018.
Article
in En
| MEDLINE
| ID: mdl-30343282
ABSTRACT
BACKGROUND:
There are limitations to current colorectal cancer (CRC)-specific diagnostic methods and therapies. Tumorigenesis proceeds because of interaction between cancer cells and various surrounding cells; discovering new molecular mediators through studies of the CRC secretome is a promising approach for the development of CRC diagnostics and therapies. MATERIALS ANDMETHODS:
A comparative secretomic analysis was performed using primary and metastatic human isogenic CRC cells. Proliferation was determined by MTT and thymidine incorporation assay, migration was determined by wound-healing assay (ELISA). The level of palmitoleoyl-protein carboxylesterase (NOTUM) in plasma from patients with CRC was determined by enzyme-linked immunosorbent assay.RESULTS:
NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. The result was verified in patients with CRC.CONCLUSION:
NOTUM may be a new candidate for diagnostics and therapy of CRC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Colorectal Neoplasms
/
Gene Expression Regulation, Enzymologic
/
Gene Expression Regulation, Neoplastic
/
Cell Proliferation
/
Esterases
/
Neoplasm Proteins
Limits:
Humans
Language:
En
Journal:
Cancer Genomics Proteomics
Journal subject:
BIOQUIMICA
/
GENETICA MEDICA
/
NEOPLASIAS
Year:
2018
Document type:
Article