Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells.

Vangapandu, Hima V; Gandhi, Varsha

Vangapandu, Hima V; Gandhi, Varsha.

Affiliation

Vangapandu HV; Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Gandhi V; Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. vgandhi@mdanderson.org.

Methods Mol Biol ; 1881: 121-128, 2019.

Article in En | MEDLINE | ID: mdl-30350202

Subject(s)

Biological Assay/methods; Leukemia, Lymphocytic, Chronic, B-Cell/pathology; Metabolic Flux Analysis/methods; Biological Assay/instrumentation; Cell Line; Cell Line, Tumor; Coculture Techniques/instrumentation; Coculture Techniques/methods; Energy Metabolism/drug effects; Fibroblasts; Glycolysis/drug effects; Humans; Leukemia, Lymphocytic, Chronic, B-Cell/blood; Leukemia, Lymphocytic, Chronic, B-Cell/metabolism; Leukocytes; Metabolic Flux Analysis/instrumentation; Mitochondria/drug effects; Mitochondria/metabolism; Oxidative Phosphorylation/drug effects; Oxygen Consumption/drug effects; Primary Cell Culture/instrumentation; Primary Cell Culture/methods; Tumor Cells, Cultured; Uncoupling Agents/pharmacology

Key words

ECAR; Extracellular flux; Metabolism; OCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Leukemia, Lymphocytic, Chronic, B-Cell / Metabolic Flux Analysis Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States

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