A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients.
Ocul Surf
; 17(1): 160-166, 2019 01.
Article
in En
| MEDLINE
| ID: mdl-30399438
ABSTRACT
PURPOSE:
To elucidate the correlation between lactoferrin concentration in the tear film and signs and symptoms of severe dry eye disease (DED) using a novel microfluidic paper-based analytical device (µPAD) and enzyme-linked immunosorbent assay (ELISA).METHODS:
Twenty-four patients were recruited at the Keio University Hospital. Using a novel µPAD, lactoferrin concentrations were measured in 4 patients with GVHD-related DED, 3 patients with other types of DED and 2 controls (Group A). For validation by ELISA, 22 patients (7 patients from Group A) comprising 9 patients with GVHD-related DED, 6 patients with other types of DED and 7 controls were examined (Group B). The link between lactoferrin concentration and clinical data about the severity of aqueous tear deficient DED was also investigated by both µPAD and ELISA.RESULTS:
The lactoferrin concentration in tear fluid of the DED patients was positively correlated between µPAD and ELISA (pâ¯=â¯0.006, râ¯=â¯0.886). The tear fluid of the GVHD patients showed low or undetectable lactoferrin concentration. Analysis by ELISA demonstrated that lactoferrin concentrations in the tear film from the GVHD patients were significantly lower than those from the non-GVHD patients (pâ¯=â¯0.010576). ELISA revealed lactoferrin concentration correlated with the value of Schirmer test and tear film breakup time, whereas it was inversely correlated with OSDI, fluorescein and rose bengal scores.CONCLUSIONS:
The novel µPAD may pave the way for measuring lactoferrin concentration in tear fluid from DED patients. Our results suggested that lactoferrin concentration in tear fluid reflect the severity of DED.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tears
/
Enzyme-Linked Immunosorbent Assay
/
Dry Eye Syndromes
/
Lactoferrin
Type of study:
Observational_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Ocul Surf
Year:
2019
Document type:
Article
Affiliation country:
Japan