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Single-Cell Transcriptomics of Human Mesenchymal Stem Cells Reveal Age-Related Cellular Subpopulation Depletion and Impaired Regenerative Function.
Khong, Sacha M L; Lee, Ming; Kosaric, Nina; Khong, Danika M; Dong, Yixiao; Hopfner, Ursula; Aitzetmüller, Matthias M; Duscher, Dominik; Schäfer, Richard; Gurtner, Geoffrey C.
Affiliation
  • Khong SML; Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
  • Lee M; Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
  • Kosaric N; Emory University School of Medicine, Atlanta, Georgia, USA.
  • Khong DM; Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
  • Dong Y; Massachusetts General Hospital, Harvard Medical School, Harvard University, Boston, Massachusetts, USA.
  • Hopfner U; Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
  • Aitzetmüller MM; Department of Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Duscher D; Department of Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Schäfer R; Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
  • Gurtner GC; Department of Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
Stem Cells ; 37(2): 240-246, 2019 02.
Article in En | MEDLINE | ID: mdl-30412645
ABSTRACT
Although bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely recognized as promising therapeutic agents, the age-related impacts on cellular function remain largely uncharacterized. In this study, we found that BM-MSCs from young donors healed wounds in a xenograft model faster compared with their aged counterparts (p < .001). Given this significant healing advantage, we then used single-cell transcriptomic analysis to provide potential molecular insights into these observations. We found that the young cells contained a higher proportion of cells characterized by a higher expression of genes involved in tissue regeneration. In addition, we identified a unique, quiescent subpopulation that was exclusively present in young donor cells. Together, these findings may explain a novel mechanism for the enhanced healing capacity of young stem cells and may have implications for autologous cell therapy in the extremes of age. Stem Cells 2019;37240-246.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesenchymal Stem Cells / Transcriptome Type of study: Prognostic_studies Limits: Adult / Aged / Animals / Humans Language: En Journal: Stem Cells Year: 2019 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mesenchymal Stem Cells / Transcriptome Type of study: Prognostic_studies Limits: Adult / Aged / Animals / Humans Language: En Journal: Stem Cells Year: 2019 Document type: Article Affiliation country: United States