Evaluation of the Mitra microsampling device for use with key urinary metabolites in patients with Alkaptonuria.
Bioanalysis
; 10(23): 1919-1932, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30412682
ABSTRACT
AIM:
Alkaptonuria is a disorder of tyrosine metabolism where elevated circulating homogentisic acid causes progressive dysfunction of collagenous tissues. Logistical, financial and patient experience concerns with collection and transport of specimens to central laboratories makes evaluation of microsampling attractive.METHODOLOGY:
Volumetric absorptive microsampling devices were evaluated for accuracy, precision and drying time. Elution methods were evaluated for several urinary metabolites of interest and stability assessed under multiple conditions. Comparison was performed between dried and liquid specimens via LC-MS/MS.CONCLUSION:
Volumetric absorptive microsampling was found to be highly accurate and precise. Elution methods showed good recovery and reproducibility. Dried and liquid samples compared favorably. Analyte stability was variable, presenting barriers to implementation into routine use in this context.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clinical Chemistry Tests
/
Alkaptonuria
Limits:
Humans
Language:
En
Journal:
Bioanalysis
Year:
2018
Document type:
Article
Affiliation country:
United kingdom