Ratiometric Fluorescent Detection of Intracellular Singlet Oxygen by Semiconducting Polymer Dots.
Anal Chem
; 90(24): 14629-14634, 2018 12 18.
Article
in En
| MEDLINE
| ID: mdl-30463405
ABSTRACT
Singlet oxygen (1O2) plays important roles in many biological processes. However, it is very difficult to detect 1O2 in the intracellular environment because of its relatively low concentration and short lifetime. Here, we developed a ratiometric probe based on semiconducting polymer dots (Pdots) that can sensitively detect 1O2 in live cells. An organic dye, singlet oxygen sensor green (SOSG), was doped in polyfluorene Pdots, and excitation energy was efficiently transferred from the polymer to the SOSG dye. Accordingly, the Pdots showed constant blue fluorescence as a reference, and increased green fluorescence upon singlet oxygen generation. The ratiometric response of Pdots was examined in the intracellular environment by in situ 1O2 generation with a photosensitizer and light irradiation. Both spectroscopic measurements and confocal imaging were performed to monitor intracellular 1O2 generation during photodynamic therapy using the Pdot probe. Our results indicate that the SOSG-doped Pdots are promising for intracellular 1O2 detection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymers
/
Microscopy, Confocal
/
Singlet Oxygen
/
Quantum Dots
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anal Chem
Year:
2018
Document type:
Article
Affiliation country:
China