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Detection of IL-1ß, IL-6 and TNF-α in Sprague-Dawely rats' atrophic thymus induced by lipopolysaccharide.
Zhong, Y B; Zhang, X L; Lv, M Y; Hu, X F; Li, Y.
Affiliation
  • Zhong YB; College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, P. R. China.
  • Zhang XL; Technology Center of Experimental Animal, Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Avenue, Wanli District, Nanchang 330004, Jiangxi, P. R. China.
  • Lv MY; College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, P. R. China.
  • Hu XF; College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, P. R. China.
  • Li Y; College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, P. R. China.
Pol J Vet Sci ; 21(3): 589-597, 2018 Sep.
Article in En | MEDLINE | ID: mdl-30468342
ABSTRACT

OBJECTIVE:

This study aimed to investigate developmental changes of the thymus and intra- thymic IL-1ß, IL-6 and TNF-α expression in weaned Sprague-Dawley rats induced by lipopolysac- charide.

METHODS:

Forty healthy weaned rats aged 26 days and weighing 83±4 g were randomly and equally divided into two groups. The lipopolysaccharide group was treated daily with a single injection of lipopolysaccharide for 10 consecutive days, and the saline group was treated with an equal volume of sterilized saline. On the 1st, 4th, 7th and 10th day, histological changes and distribu- tion of IL-1ß-, IL-6- and TNF-α-positive cells were detected in the thymus by hematoxylin-eosin and immunohistochemistry staining, respectively. Subsequently, the expression levels of IL-1ß, IL-6 and TNF-α were evaluated in the thymus by the ELISA method.

RESULTS:

Thymus weight and index were significantly smaller in lipopolysaccharide-treated rats than in saline-treated rats (p⟨0.05), but no substantial changes were found in the thymus microstructure after lipopolysaccharide induction. Moreover, a large number of IL-1ß-, IL-6- and TNF-α-positive cells were observed with brownish-yellow color and mainly distributed in the thy- mus parenchyma, both integrated optical density and average optical density increased signifi- cantly in lipopolysaccharide-treated rats than those in saline-treated rats. Compared with the saline group, most of the thymic homogenates had higher levels of IL-1ß, IL-6 and TNF-α in the lipopolysaccharide group on different days.

CONCLUSION:

These findings indicate that the thymus atrophied after lipopolysaccharide induction in weaned Sprague-Dawley rats, and excessive production of intrathymic IL-1ß, IL-6 and TNF-α was probably involved in the atrophic process.
Subject(s)
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Thymus Gland / Lipopolysaccharides / Interleukin-6 / Tumor Necrosis Factor-alpha / Interleukin-1beta Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Pol J Vet Sci Journal subject: MEDICINA VETERINARIA Year: 2018 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Thymus Gland / Lipopolysaccharides / Interleukin-6 / Tumor Necrosis Factor-alpha / Interleukin-1beta Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Pol J Vet Sci Journal subject: MEDICINA VETERINARIA Year: 2018 Document type: Article