Geographical origin and species differentiation of Transylvanian cheese. Comparative study of isotopic and elemental profiling vs. DNA results.
Food Chem
; 277: 307-313, 2019 Mar 30.
Article
in En
| MEDLINE
| ID: mdl-30502149
ABSTRACT
The present study proposed the cheese differentiation, according to geographical production area and with respect to species (cow, sheep) of two traditional cheese specialties, (salty and ripened), produced in Transylvania, Romania. For this purpose, the elemental profile and carbon isotopic ratios (13C/12C) of cheese and extracted casein were corroborated through statistic supervised techniques to get the best discrimination markers. The manganese content, along with Rare Earth Elements (REE) concentrations, proved to be very powerful predictors, for the traditional salted cheese mainly, due to the direct influence of the local salted water. Despite that proposed techniques are not acknowledged methods for species differentiation, this approach allowed a successful discrimination of the animal species that produced the raw materials for cheese manufacturing (milk). The results generated by the developed chemometric model, for species differentiation, were compared with those obtained using Isoelectric focusing (IEF) and DNA tests. The proposed association of isotopic and elemental markers allowed a differentiation better than 92% for geographical provenance, of each investigated cheese specialties while, for species discrimination (cow vs. sheep) a percentage of 100% was obtained.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trace Elements
/
DNA
/
Carbon Isotopes
/
Cheese
/
Metals, Rare Earth
Type of study:
Prognostic_studies
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Food Chem
Year:
2019
Document type:
Article