An inexpensive system for imaging the contents of multi-well plates.
Acta Crystallogr F Struct Biol Commun
; 74(Pt 12): 797-802, 2018 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-30511674
ABSTRACT
An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or movies though a microscope-mounted digital camera. The translation stage provides reproducible three-dimensional movement of the sample over a volume of 160â
mm in x, 100â
mm in y and 40â
mm in z. A Python script generates the G-code command file that scans the plate and collects a series of z-stacked images of each sample. A second Python script automates the calculation of images with a digitally enhanced depth of field. The imaging system is currently being used to facilitate screening for protein crystals, but it could be used to automate the imaging of many other types of samples in multi-well plates.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cost-Benefit Analysis
/
Imaging, Three-Dimensional
/
Microscopy
Language:
En
Journal:
Acta Crystallogr F Struct Biol Commun
Year:
2018
Document type:
Article
Affiliation country:
United States