Novel approach to improve progesterone hydroxylation selectivity by CYP106A2 via rational design of adrenodoxin binding.

Sagadin, Tanja; Riehm, Jan; Putkaradze, Natalia; Hutter, Michael C; Bernhardt, Rita

Sagadin, Tanja; Riehm, Jan; Putkaradze, Natalia; Hutter, Michael C; Bernhardt, Rita.

Affiliation

Sagadin T; Department of Biochemistry, Saarland University, Saarbrücken, Germany.
Riehm J; Center for Bioinformatics, Saarland University, Saarbrücken, Germany.
Putkaradze N; Department of Biochemistry, Saarland University, Saarbrücken, Germany.
Hutter MC; Center for Bioinformatics, Saarland University, Saarbrücken, Germany.
Bernhardt R; Department of Biochemistry, Saarland University, Saarbrücken, Germany.

FEBS J ; 286(6): 1240-1249, 2019 03.

Article in En | MEDLINE | ID: mdl-30537187

Subject(s)

Adrenodoxin/metabolism; Bacillus megaterium/metabolism; Bacterial Proteins/metabolism; Cytochrome P-450 Enzyme System/metabolism; Progesterone/metabolism; Adrenodoxin/chemistry; Adrenodoxin/genetics; Bacillus megaterium/genetics; Bacillus megaterium/growth & development; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Cytochrome P-450 Enzyme System/chemistry; Cytochrome P-450 Enzyme System/genetics; Hydroxylation; Models, Molecular; Molecular Docking Simulation; Mutation; Oxidation-Reduction; Protein Conformation

Key words

Adrenodoxin; CYP106A2; cytochrome P450; electron transfer; molecular dynamics simulation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Progesterone / Bacillus megaterium / Bacterial Proteins / Adrenodoxin / Cytochrome P-450 Enzyme System Language: En Journal: FEBS J Journal subject: BIOQUIMICA Year: 2019 Document type: Article Affiliation country: Germany Country of publication: United kingdom

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